RESOURCES

Protocols

Positive selection of GC B cells using anti-DEC-OVA

Pasqual G, Angelini A, Victora GD. Triggering positive selection of germinal center B cells by antigen targeting to DEC-205. Methods Mol Biol. 2015;1291:125-34. doi: 10.1007/978-1-4939-2498-1_10

In situ photoactivation for anatomically-defined flow cytometry

Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation. Jacobsen JT, Victora GD. Methods Mol Biol. 2017;1623:51-58. doi: 10.1007/978-1-4939-7095-7_4

Mouse Strains

PA-GFP-transgenic

This strain has ubiquitous expression of PA-GFP. Randomly inserted transgene using the human ubiquitin C promoter. Useful for photoactivation of cells on a two-photon microscope for downstream flow cytometry or tracking in vivo. Described here. Available from Jax

 

Cd40G5 and Cd40SrtA (LIPSTIC)

This pair of strains is the basis of our first published version of the LIPSTIC technology. These mice can be used to label interactions between T cells and dendritic cells in vivo. Described here. Available by request.

MtorF2108L (rapamycin-resistant)

This strain carries a point mutation derived from a human tumor that confers resistance to rapamycin. Useful for testing cell-intrinsic roles of mTORC1 (transfer WT cells into F2108L hosts, or vice-versa). Described here and here. Available by request.

Plasmids

LIPSTIC plasmids

Collection of plasmids encoding for different LIPSTIC components for transient expression in cell lines or retroviral transduction of primary lymphocytes. Includes the cognate pair of G5-CD40 and CD40L-SrtA as well as a control membrane SrtA. Available from Addgene

Igh bicistronic knock-in

Targeting vector for knocking in antibody heavy and light chains bicistronically into the Igh locus by mouse zygote CRISPR, as described here. Available upon request. 

© 2020 by VICTORA LAB, The Rockefeller University