LAB MEMBERS
LAB MEMBERS
LAB MEMBERS
LAB MEMBERS
RESOURCES
PROTOCOLS
LIPSTIC labeling for flow cytometry
Monitoring the Interaction Between Dendritic Cells and T Cells In Vivo with LIPSTIC. Pasqual G, Chudnovskiy A, Victora GD.Methods Mol Biol. 2023;2618:71-80. doi:10.1007/978-1-0716-2938-3_5
Intravital imaging
In Vivo Imaging of Tfh Cells. Nakandakari-Higa S, Jacobsen JT.Methods Mol Biol. 2022;2380:15-27. doi:10.1007/978-1-0716-1736-6_2
Positive selection of GC B cells using anti-DEC-OVA
Triggering positive selection of germinal center B cells by antigen targeting to DEC-205. Pasqual G, Angelini A, Victora GD. Methods Mol Biol. 2015;1291:125-34. doi:10.1007/978-1-4939-2498-1_10
In situ photoactivation for anatomically-defined flow cytometry
Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation. Jacobsen JT, Victora GD. Methods Mol Biol. 2017;1623:51-58. doi:10.1007/978-1-4939-7095-7_4
MICE
PA-GFP-transgenic
This strain has ubiquitous expression of PA-GFP. Randomly inserted transgene using the human ubiquitin C promoter. Useful for photoactivation of cells on a two-photon microscope for downstream flow cytometry or tracking in vivo. Described here. Available from Jax.
Cd40^G5 and Cd40^SrtA (LIPSTIC)
This pair of strains is the basis of our first published version of the LIPSTIC technology. These mice can be used to label interactions between T cells and dendritic cells in vivo. Described here, here, and here. Available from Jax.
Mtor^F2108L (rapamycin-resistant)
This strain carries a point mutation derived from a human tumor that confers resistance to rapamycin. Useful for testing cell-intrinsic roles of mTORC1 (transfer WT cells into F2108L hosts, or vice-versa). Described here and here. Available by request.
Igk^Tag (molecular fate-mapping of antibodies)
Described here. Available from Jax.
Rosa26^uLIPSTIC (Universal LIPSTIC donor/acceptor)
PLASMIDS
LIPSTIC plasmids
Collection of plasmids encoding for different LIPSTIC components for transient expression in cell lines or retroviral transduction of primary lymphocytes. Includes the cognate pair of G5-CD40 and CD40L-SrtA as well as a control membrane SrtA. Available from Addgene.
Igh bicistronic knock-in
Targeting vector for knocking in antibody heavy and light chains bicistronically into the Igh locus by mouse zygote CRISPR, as described here. Available upon request.