LAB MEMBERS
LAB MEMBERS
LAB MEMBERS
LAB MEMBERS
RESOURCES
Protocols
LIPSTIC labeling for flow cytometry
Monitoring the Interaction Between Dendritic Cells and T Cells In Vivo with LIPSTIC. Pasqual G, Chudnovskiy A, Victora GD.Methods Mol Biol. 2023;2618:71-80. doi:10.1007/978-1-0716-2938-3_5
Intravital imaging
In Vivo Imaging of Tfh Cells. Nakandakari-Higa S, Jacobsen JT.Methods Mol Biol. 2022;2380:15-27. doi:10.1007/978-1-0716-1736-6_2
Positive selection of GC B cells using anti-DEC-OVA
Triggering positive selection of germinal center B cells by antigen targeting to DEC-205. Pasqual G, Angelini A, Victora GD. Methods Mol Biol. 2015;1291:125-34. doi:10.1007/978-1-4939-2498-1_10
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In situ photoactivation for anatomically-defined flow cytometry
Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation. Jacobsen JT, Victora GD. Methods Mol Biol. 2017;1623:51-58. doi:10.1007/978-1-4939-7095-7_4
Mouse Strains
PA-GFP-transgenic
This strain has ubiquitous expression of PA-GFP. Randomly inserted transgene using the human ubiquitin C promoter. Useful for photoactivation of cells on a two-photon microscope for downstream flow cytometry or tracking in vivo. Described here. Available from Jax.
Cd40G5 and Cd40SrtA (LIPSTIC)
This pair of strains is the basis of our first published version of the LIPSTIC technology. These mice can be used to label interactions between T cells and dendritic cells in vivo. Described here, here, and here. Available from Jax.
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MtorF2108L (rapamycin-resistant)
This strain carries a point mutation derived from a human tumor that confers resistance to rapamycin. Useful for testing cell-intrinsic roles of mTORC1 (transfer WT cells into F2108L hosts, or vice-versa). Described here and here. Available by request.
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IgkTag (molecular fate-mapping of antibodies)
Described here. Available from Jax.
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Rosa26uLIPSTIC (Universal LIPSTIC donor/acceptor)
Plasmids
LIPSTIC plasmids
Collection of plasmids encoding for different LIPSTIC components for transient expression in cell lines or retroviral transduction of primary lymphocytes. Includes the cognate pair of G5-CD40 and CD40L-SrtA as well as a control membrane SrtA. Available from Addgene.
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Igh bicistronic knock-in
Targeting vector for knocking in antibody heavy and light chains bicistronically into the Igh locus by mouse zygote CRISPR, as described here. Available upon request.